اثر محافظتي تمرين هوازي همراه با مكمل كپسايسين بر شاخص هاي آپوپتوز قلب موش هاي صحرايي تغذيه شده با رژيم غذايي پرچرب

Protective Effect of Aerobic Training Along with Capsaicin Supplementation on Factors of of Cardiac Apoptosis in Rat Fed a High-Fat Diet

مقدمه مصرف رژيم غذايي پر چرب باعث چاقي ناشي از رژيم غذايي شده و با چندين خطر-قلبي عروقي از جمله سندرم متابوليكي و آپوپتوز همراه است. هدف از پژوهش حاضر بررسي اثر تمرين هوازي همراه با كپسايسين بر تنظيم كننده آپوپتوزي BAX (Bax)، لنفوم سلول B2 (Bcl2) و نسبت Bax/BCL2 قلبي در موش هاي تغذيه شده با رژيم غذايي پرچرب (HFD) مي باشد. مواد و روش ها 40 سر موش صحرايي نر ويستار به مدت 8 هفته با رژيم غذايي نرمال (8=n، ND) و HFD (32=n) تغذيه شدند. بعد از هشت هفته همه موش ها به 5 گروه: ND، HFD، رژيم غذايي پرچرب-تمرين (HFDT)، رژيم غذايي پرچرب-كپسايسين (HFDCap) و رژيم غذايي پرچرب-تمرين- كپسايسين (HFDTCap) تقسيم شدند. گروه هاي تمرين به مدت هشت هفته برنامه تمرين هوازي فزاينده (25-15 متر در دقيقه، 60-30 دقيقه در روز، پنج روز هفته) را روي تردميل انجام دادند. كپسايسين (mg/kg/day 4) يك بار در روز به صورت خوراكي با گاواژ خورانده شد. ميزان Bax، Bcl2 بافت قلب به روش الايزا اندازه گيري شد. يافته ها نتايج پژوهش حاضر نشان دهنده كاهش Bax و نسبت Bax/Bcl2 در گروه هاي HFDT، HFDCap و HFDTCap نسبت به HFD بود (0/05

Introduction High-fat diet intake leads to diet-induced obesity in concert with multiple cardiovascular risk factors such as metabolic syndrome and apoptosis. The aim of this study was to evaluate the effects of aerobic training with along capsaicin on cardiac apoptosis regulator BAX (Bax), B- cell lymphoma 2 (Bcl2) and Bax/Bcl2 in rats fed a high-fat diet (HFD). Materials and Methods 40 male Wistar rats were fed a normal diet (ND, n = 8) or HFD (n = 32) for 8 weeks. After 8 weeks, all rats were divided into 5 groups: ND, HFD, high-fat diet-training (HFDT), high-fat diet-capsaicin (HFDCap), high-fat diet-trainingcapsaicin (HFDTCap). Training groups have performed a progressive aerobic running program (at 15-25 m/min, 30-60 min/day, and 5 days/week) on a motor-driven treadmill for eight weeks. Capsaicin (4 mg/kg/day) was administered orally, by gavage, once a day. Results The results of this study showed that Bax and Bax/Bcl2 in HFDT (Respectively p=0.016, p=0.000), HFDCap (Respectively p=0.014, p=0.000) and HFDTCap (Respectively p=0.000, p=0.000) groups was significantly decreased compared to HFD. Bax and Bax/Bcl2 in HFDTCap was significantly decreased compared to HFDT (Respectively p=0.034, p=0.033) and HFDCap (Respectively p=0.038, p=0.036) groups. Also, Bcl2 significantly increased in to HFDT (p=0.001) and HFDCap (p=0.000) and HFDTCap (p=0.000) groups compared to HFD. Conclusion Exercise and capsaicin improve the apoptosis induced by obesity and suggest that exercise and capsaicin can be considered as an excellent candidate for therapy of these clinical conditions.