كاهش اثرات استرس حاد بر كيفيت اسپرم منجمد-يخ گشايي شده خروس با افزودن عصاره هاي هيدروالكي رزماري و رازيان ه در رقيق كننده انجماد

Reducing the effects of acute stress on frozen-thawed rooster’s semen quality with supplementation of herbal extracts in cryopreservation extender

ﻫﺪف اﯾﻦ آزﻣﺎﯾﺶ ﺑﺮرﺳﯽ اﻓﺰودن ﻋﺼﺎرهﻫﺎي ﻫﯿﺪرواﻟﮑﻠﯽ ﮔﯿﺎﻫﺎن رزﻣﺎري و رازﯾﺎﻧﻪ در رﻗﯿﻖﮐﻨﻨﺪه ﺑﺮ ﮐﯿﻔﯿﺖ اﺳﭙﺮم ﻣﻨﺠﻤﺪ-ﯾﺦﮔﺸﺎﯾﯽ ﺷﺪه ﺧﺮوسﻫﺎي ﺗﺤﺖ ﺗﻨﺶ ﺣﺎد اﮐﺴﯿﺪاﺗﯿﻮ ﺑﻮد. 24ﻗﻄﻌﻪ ﺧﺮوس ﺳﻮﯾﻪ راس 308ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ در دو دﺳﺘﻪ ﺑﺪون ﺗﻨﺶ )ﮐﻨﺘﺮل( و ﺗﺤﺖ ﺗﺄﺛﯿﺮ ﺗﻨﺶ اﮐﺴﯿﺪاﺗﯿﻮ ﺑﺎ ﺗﺰرﯾﻖ دﮔﺰاﻣﺘﺎزون ﺑﻪ ﺻﻮرت زﯾﺮﺟﻠﺪي در ﺳﻪ ﻧﻮﺑﺖ ﺑﺼﻮرت ﯾﮏ روز در ﻣﯿﺎن و ﺑﻪ ﻣﻘﺪار 4 ﻣﯿﻠﯽﮔﺮم ﺑﻪ ازاي ﻫﺮ ﮐﯿﻠﻮﮔﺮم وزن ﺑﺪن در ﯾﮏ ﻫﻔﺘﻪ )دﮔﺰا( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﻣﻨﯽ ﮔﺮﻓﺘﻪ ﺷﺪه ﭘﺮﻧﺪﮔﺎن ﻫﺮ ﮔﺮوه ﻣﺨﻠﻮط و ﺑﺮاي اﻋﻤﺎل ﺑﺮﻧﺎﻣﻪ اﻧﺠﻤﺎدي، ﺑﻪ ﻋﻨﻮان ﮔﺮوهﻫﺎي آزﻣﺎﯾﺸﯽ ﺑﻪ اﯾﻦ ﺷﺮح اﺳﺘﻔﺎده ﺷﺪ: 1( ﮔﺮوه ﮐﻨﺘﺮل )ﺑﺪون اﺳﺘﺮس و ﻣﮑﻤﻞ ﺳﺎزي رﻗﯿﻖﮐﻨﻨﺪه ﺑﺎ ﻋﺼﺎره(،2 ( ﮔﺮوه درﯾﺎﻓﺖﮐﻨﻨﺪه دﮔﺰاﻣﺘﺎزون )دﮔﺰا(،3 ( ﮔﺮوه ﻋﺼﺎره ﻫﯿﺪرواﻟﮑﻠﯽ رزﻣﺎري ﺑﻪ ﻣﻘﺪار 10ﻣﯿﻠﯽﮔﺮم در دﺳﯽﻟﯿﺘﺮ رﻗﯿﻖﮐﻨﻨﺪه ﮔﺮوه ﮐﻨﺘﺮل )ﮐﻨﺘﺮل+رزﻣﺎري(،4 ( ﮔﺮوه ﻋﺼﺎره ﻫﯿﺪرواﻟﮑﻠﯽ رزﻣﺎري ﺑﻪ ﻣﻘﺪار 10ﻣﯿﻠﯽﮔﺮم در دﺳﯽﻟﯿﺘﺮ رﻗﯿﻖﮐﻨﻨﺪه ﮔﺮوه دﮔﺰاﻣﺘﺎز ون )دﮔﺰا+رزﻣﺎري(،5 ( ﮔﺮوه ﻋﺼﺎره ﻫﯿﺪرواﻟﮑﻠﯽ رازﯾﺎﻧﻪ ﺑﻪ ﻣﻘﺪار 6ﻣﯿﻠﯽﮔﺮم در دﺳﯽﻟﯿﺘﺮ رﻗﯿﻖﮐﻨﻨﺪه ﮔﺮوه ﮐﻨﺘﺮل )ﮐﻨﺘﺮل+رازﯾﺎﻧﻪ( و6 ( ﮔﺮوه ﻋﺼﺎره ﻫﯿﺪرواﻟﮑﻠﯽ رازﯾﺎﻧﻪ ﺑﻪ ﻣﻘﺪار 6ﻣﯿﻠﯽﮔﺮم در دﺳﯽﻟﯿﺘﺮ رﻗﯿﻖﮐﻨﻨﺪه ﮔﺮوه دﮔﺰاﻣﺘﺎزون )دﮔﺰا+رازﯾﺎﻧﻪ(. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﺗﻨﺶ اﯾﺠﺎدﺷﺪه ﻣﻮﺟﺐ ﮐﺎﻫﺶ ﻓﺮآﺳﻨﺠﻪﻫﺎي ﺣﺮﮐﺘﯽ و ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ اﺳﭙﺮم ﺷﺪ )0/05>P(. زﻧﺪهﻣﺎﻧﯽ و ﯾﮏﭘﺎرﭼﮕﯽ ﻏﺸﺎي اﺳﭙﺮم در ﮔﺮوهﻫﺎي ﮐﻨﺘﺮل+رزﻣﺎري و ﮐﻨﺘﺮل+رازﯾﺎﻧﻪ ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ ﮔﺮوهﻫﺎي آزﻣﺎﯾﺸﯽ ﺑﺎﻻﺗﺮ ﺑﻮد )0/05>P(، ﻫﻤﭽﻨﯿﻦ اﻓﺰودن ﻋﺼﺎرهﻫﺎي رزﻣﺎري و رازﯾﺎﻧﻪ در ﮔﺮوهﻫﺎي درﯾﺎﻓﺖﮐﻨﻨﺪه دﮔﺰا، ﻣﻮﺟﺐ ﺑﻬﺒﻮد ﻓﺮآﺳﻨﺠﻪﻫﺎي ﻣﺬﮐﻮر و ﻧﯿﺰ ﺗﺤﺮك ﮐﻞ و ﭘﯿﺶروﻧﺪه در اﺳﭙﺮم ﭘﺮﻧﺪﮔﺎن ﻣﺬﮐﻮر ﮔﺮدﯾﺪ )0/05>P(. ﺑﻪ ﻃﻮر ﮐﻠﯽ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﻣﮑﻤﻞﺳﺎزي رﻗﯿﻖﮐﻨﻨﺪه اﺳﭙﺮم ﺑﺎ ﻋﺼﺎرهﻫﺎي رزﻣﺎري و رازﯾﺎﻧﻪ ﻣﯽﺗﻮاﻧﺪ ﻣﻮﺟﺐ ﺑﻬﺒﻮد ﮐﯿﻔﯿﺖ اﺳﭙﺮم ﻣﻨﺠﻤﺪ-ﯾﺦﮔﺸﺎﯾﯽ ﺧﺮوسﻫﺎي ﭘﺮورش ﯾﺎﻓﺘﻪ ﺗﺤﺖ تنش ﺣﺎد ﮔﺮدد.

The aim of this study was evaluation of cryopreservability of rooster semen supplemented with Rosemary and Fennel extracts in extender on quality of cryopreserved sperm under acute stress. A total of 24 roosters randomly divided into two groups: without stress (CON) or under oxidative stress, with administration of dexamethasone, 4 mg/Kg body weight, three times, every other day for 1 week, (DEX). Collected semen samples of birds were pooled in each groups and used in one of following treatments: 1) CON: with no extract supplementation or dexamethasone administration, 2) DEX: Dexamethasone group, 3) CON+Ros: semen of control group supplemented with 10 ml rosemary extract per deciliter of extender, 4) DEX+Ros; semen of DEX group supplemented with 10 ml rosemary extract per deciliter of extender, 5) CON+Fen: semen of control group supplemented with 6 ml fennel extract per deciliter of extender and 6) CON+Fen: semen of DEX group supplemented with 6 ml fennel extract per deciliter of extender. Results showed induced stress decreased sperm motility and biochemical parameters (P<0.05). Viability and membrane integrity of sperm were higher in CON+Ros and CON+Fen than other experimental groups (P<0.05). Similarly, supplementation of extender with rosemary and fennel extract in groups DEX+Fen and DEX+Ros improved mentioned parameters in rooster’s semen under physiologic stress. Totally, results showed supplementation of rosemary and fennel extract in cryopreservation extender can improve the quality of frozen-thawed semen in roosters reared under physiologic stress.