تغييرات فاكتورهاي ايمني در ورزشكاران مرد پس از يك فعاليت شديد ورزشي

Changes of the immune system in male athletes after a heavy athletic activity

The present research studies changes resulting from regular and continuos exercises as well as the effect of a heavy aerobic activity on the immune system of athletes. In this investigation 15 professional athletes from different sports clubs and universities along with 15 non-athlete students whom were matched for sex, age, weight and height were selected. Each individual completed a special questionnaire to he healthy during the study period. Their immune status was evaluated by assessment of the following parameters: WBC differential for lymphocytes, neutrophils, monocytes and eosinophils. Immunophenotyping by flow cytometry was performed using the following markers: CD3 for T- cell,CDI9 for B-cell, CDI4 for monocyte, CD56for NK-cell, CD45 far leuckocyte, CD,/ for T-h, CD8 for T-s/c &CD4/CD8 ratio. Also IgG was determined by SRID(single radial immunodiffusion). The statistical community of this research was Tehran University. The proposed activity, a heavy aerobic exercise activity (Bruce protocol), was carried out on a treadmill and continued until the border of incapability. A sample of 10ml blood was obtained from each individual of non-athletes group at rest position between 8:30-9:00 am. The athletes group were also Needed the same volume, and at the same time (30 minutes before starting Bruce test), and then, the second blood sample (10ml) was obtained from each athlete, immediately after the test. Blood samples were sent to immunogenetic laboratory for flow cytometrv, hematology and h immunoglobulin assay test. Results revealed that a heavy aerobic activity can decrease the amount of IgG and increase the number cif NK cells (CD36) following Bruce treadmil test in professional athletes compared with non-athletes. Also, a significant increase of CD4 and CD8 cells was observed in athletes group.