مروري بر گونه هاي ورتيسيليوم مرتبط با گياهان

A REVISION OF PLANT-ASSOCIATED VERTICILLIUM SPECIES

Molecular approaches were used to re-evaluate the morphological criteria used to identify plant-associated Verlicilliran species. ITS-RFLPs divided the 31 studied strains of seven Vert/el/hum species, (including the type species V luteo-album) into four clusters. Cluster one comprised strains of the type species, V. /oleo-album, cluster two V. albo-atrum, V. dahliae, V nubilutn and V. tricorpus; cluster three comprised strains of V. theobromae, and cluster four comprised strains of V. nigrescens. 3-tubulin gene RFLPs offered a higher degree of resolution, distinguishing all seven species from each other. The highest degree of resolution was obtained from mitochondria) DNA-RFLPs that divided strains of V. theobromae and V. nigrescens into infraspecific groups. The f3-tubulin gene digested by Hae III offers a reliable way to separate the two economically important and controversial species V albo-atrunr and V dahliae.