با كاربرد ژل كروماتوگرافي ، روش ضد قارچي و طيف سنجي A بررسي تخمير، جداسازي و شناسايي سيكلوسپورين

Assesment, isolation and identification of cyclosporin A by gel chromatography, fungicidal activity and spectroscopy

Introduction: T.inflatum (Tolypocladium inflatum) is a primitive fungi that reproducts via conidiums. Spors grow up on malt extract culture at 6-32°C at wet or dry medium. Of course it is found of some others culture which are contain aminoacids, mineral or organic matter. Cyclosporin A (Cy A) is the metabolite of t.intlatum. Cy A is an immunosuppressive drug. The aim of this study was to promote production of Cy A by mutation via ultraviolet radiation (UVR) and to find out a suitable medium for auxotrophs. Material and Methods: Tolypocladium inflatum (DSM 915), malt extract and sabourd 4% dextrose agar, Bactopeptamine, casein hydrolysate and yeast extract dehydrated. The fungi was cultivated for 10-20 days duration on a medium contains 2% malt extract, 4% yeast extract and 20% agar at pH 5.7. Extraction was carried out by gel chromatography. For mutation of the fungi, UVR was used, and for identification of Cy A fungicidal, ultraviolet, visible and infra-red spectroscopy were methods of choice. Results: White velvey colonies were collected from the medium of agar and malt extract. Samples and fractions of gel chromatography extraction showed antifungal activity on aspergillus niger. Ultraviolet visible spectroscopy gave>lmax similar to that of standared and infra-red spectra was similar to the standared spectra. Conclusion: Auxotrophic strains were cultivated on synthetic and raw mediums. Finally the high production of Cy A was produced of sugar factroy and hogwash of corn oil pressing factroy, at a dark place.