پلاسمايي به هنگام تزريق آهن دكستران در موشهاي صحرايي نر E اثر مهاري تيتريك اكسايد در جلوگيري از كاهش ويتامين

INHIBITORY EFFECT OF NITRIC OXIDE IN PREVENTING PLASMA VITAMIN E REDUCTION DURING IRON DEXTRAN INJECTION IN MALE RATS

Background & aim: While the role of iron in induction of oxidative stress has been confirmed, the Nitric Oxide oneʹs (NO) remained vague. Also there have been some controversies on the interaction of iron and NO. In this study we have examined this interaction in plasma and we measured Vit E (Vitamin E) level as a marker of oxidative stress. Material & method: Sixty-four male rats were allocated into 8 groups as the following: I- SHAM (Normal Saline), 2- Fe (Iron Dextran), 3- ARG (L-Arginine - precursor of NO), 4- L-NAME (blacker of NO synthase, 5- Fe+ARG, 6- Fe+L-NAME, 7- DFO (Defferoxamine- Iron Shelator), and 8- ARG+DFO. After 20 hours of injections, Vit E in plasma was measured by using high performance liquid chromatography. Results: Iron caused Vit E reduction in Fe group compared to SHAM group (p< 0.01). ARG did not have any effects on Vit E by itself. In Fe+ARG group there was no significant difference with sham group. The most reduction in Vit E occurred in group Fe+L-NAME so that its significant difference with SHAM and Fe+ARG was p<0.001 and 0.01 respectively. Discussion: We conclude that ARG by increasing NO concentration prevents and L-NAME by decreasing NO concentration exacerbates iron induced oxidative stress.