بررسي اثرات تراتوژنيك كلريد مس بر قشر مغز جنين موش سوري

THE COPPER CHLORIDE EFFECTS TERATOGENIC ON THE CEREBRAL CORTEX OF MOUSE EMBRYOS

Background & Aim : Copper is an essential trace metal, that it is absorbed in digestive system. Copper chloride is one of the most important of copper components. The lack of copper resulted to structural and metabolic defects. According to recent studies, copper has toxic effect on central nervous system. In spite of a little amount of copper which is necessary for brain development, but high amount of it may causes defects in CNS. Materials & Methods: We divided, 42 pregnant mice in seven groups: Three groups of experimental, three groups of control and one group of intact. Each group had six pregnant mice. Experimental group was injected 5mg/kg CuCl2 on 7th ,8th and 9th gestational days via intraperitoneal(IP). Control group was injected distilled water on the same days. Intact group was not injected. Embryos were extruded from the uterus on 15th gestational days. Weight and number of absorbed embryos were measured. Following tissue passage and sectioning, sections were stained with H & E. Results: According to macroscopic measurement, weight of embryos in all experimental groups was decreased significantaly (p<0.05). In microscopic observation, cellular irregularity and Gliosis were seen. In morphometric evaluations, in all groups, thickness of cerebral cortex was decreased (p<0.05). Our findings shows that high amount of copper chloride can caused anomaly in central nervous system.