ايمن سازي موش ها با آنتي ژي سونيكه هليكوباكتر فليس و مقايسه پاسخ هاي ايمني آن با موش هاي غير ايمن آلوده با هليكوباكتر زنده

IMMUNIZATION OF MICE WITH SONICATED ANTIGEN AGAINST HELICOBACTER FELTS AND COMPARING THE EMERGED IMMUNE RESPONSE WITH A NON IMMUNE H. FELTS INFECTED MICE

Background & Aims: The role of immunization of mice with sonicated antigen in protection of Helicobacter induced gastritis and invitro survey of lymphocyte response in immunized/ challenged mice in compare with infected mice was studied. Materials & Methods: Two groups of mice were immunized with 3 mg of sonicated antigen and along with the 2 groups of naive (non-immunized) mice were challenged by alive H.felis. The lymphocyte proliferation index, were determined by dividing the counts obtained from Ag-stimulated cells by counting from unstimulated cell/well. The assay of 1FN- y and anti-H.felis antibodies were done by sandwich ELISA. Results: Eight weeks after challenge, the lymphocyte stimulation index in infected and Immunized/challenge mice were respectively 45&25.IFN-y production due to Immune/challenge and infected mice were respectively 300 and 170 Picogram/ml. The antibodies titer of Immune/challenge and infected mice obtained 1:8000 and 1:500 respectively. Discussion: The data showed that cellular response of immunized mice were significantly higher (p<0.05) than infected mice, early after challenge. Whereas, declined within 8 weeks in Immunized mice in comparsion with infected mice. On the other hand, the higher production of IFN-ʹy and anti-H. fells antibody by immunized mice, compared with infected mice, suggest that specific response, mediated by Thl and then Th2 (via antibody production) play a role against Helicobacter-induced gastritis.