جداسازي ليشمانياميجر به روش ايزوآنزيم الكتروفورزيس در شهر مشهد

Isolation of Leishmania major by Isoenzyme Electrophoresis Method in Mashhad

Introduction: According WHO reports, Iran is recognized as an important foci for leishmaniesis in the world. This disease caused by different species of leishmania and identification of these species is useful for control and prevenation of the disease. The phenotypic characters of causative organisms are similar, also epidemiological and clinical evidences alone, can not account for the differentiation of species. Molecular techniques, most notably isoenzym electrophoresis, have revolutionized the identification of this parasite. Material and Methods: This description study in 1378 - 1379 was done in Mashhad indispite Mashhad had been considered as foci of L. tropica, but recently rural forms also have been reported. Then, we decide to identify the leishmania species in Mashhad. For the first time, to obtain precise data on leishmania species indigenous to the city of Mashhad, 18 stocks isolated from the sores of patients suffering from CL from south - west part of the city, were compared between themselves and with reference strains of L. tropica and .L. mojor. The comparison was made by examination of the electrophoresis pattern of 6 enzymes; GPI. MDH, G6PD, PGM, NHI, NH2 using polyacrylamide gel electrophoresis. Results: The isolates from cutaneous lesions fell clearly in one group. Conclusion: They all gave isoenzyme patterns identical to those of reference strains of L. major. Then against previously in had been taught, not only L. tropica but also L. major specially in outskirts of city is responsible for cutaneous leshmaniasis in Mashhad.