مقايسه روش ايميونوفلورسنت مونوكلونال آنتي بادي مستقيم و رنگ آميزي كاينيون اسيدناست در تشخيص آزمايشگاهي كريپتوريديوزيس

A COMPARATIVE STUDY ON THE TWO METHODS OF DIRECT FLUORESCENTANTIBODY AND KYNION, ACID-FAST STAINING TECHNIQUES ON THE LABORATORY DIAGNOSIS OF CRYPTOSPORIDIOSIS

Cryptosporidium parvum in humans,as a re-appeared and as one of the four most important ubiquitous enteric pathogen which can cause diarrhea with high level of mortality mainly in the Immunocompromised patients and has stimulated research interest in the world. The quick and accurate laboratory diagnostic report of the pathogen are highly important in the prevention of the disease progress. Although kynion Acid-Fast(AF)staining procedure is accepted in the most clinical laboratories,a comparative study on the preferences of Direct Fluorescent (DF) antibody with staining techniques has undertaken in this recent work.During.months,beginning 1 /5/ 1999,the stool samples of 340 apparently healthy people, 185 from patients under chemotherapy and 1 70 from children under 10 years with chronic diarrhea have been collected for our research studies .The obtained results are as following:1-The sensitivity,specificity and the simplicity of the Direct Fluorescent (DF) was 100% as compared with kynion Acid-Fast (AF) technique. 2-With regard of minimum detection time unit,each (DF) slide needs one minute, while this time limit for each (AF) slide was 5 minutes.3-By comparing the minimum diagnostic level(MDL) by expert technologists in two techniques, it was 8 for (DF). In other words, at least 60000 oocysts/ml of liquid stools are needed until to be able to detect by (AF) while (MDL) for (DF) was 7500 oocysts/ml.4-By using a single blind test the experience quality of the technologists in two techniques of (DF) and (AF) were evaluated. This test was significantly prefered as compared with (AF) technique.5-In these studies we have found that 0.7% of healthy people ,0.5% of patients under chemotherapy and 4.1% of diarrhaetic children were infected with Cryptosporidium parvum.