تاثير گلوتاتيون بر از سرگيري ميوز، بلوغ و تكوين آزمايشگاهي تخمك هاي نارس موش

Ect of Glutathion on resumpsion of Meiosi in-vitro Maturation, and development of immature Mouse oocytes

Background and purpose : In this study the effects of glutathion on resumption of meiosis, in-vitro maturation and development of immature mouse oocytes was evaluated. Materials and methods : Immature oocytes recoverd from NMR1 mouse strain (4-6 weeks) in four different experimental groups. Group 1 (control group): 345 immature oocytcs were matured in MEM-a medium containing FCS 5%, group I: 338 immature oocytes were cultured in MEM-(Alfa) medium containing FCS 5% and 1mM glutathione, group 2: 237 immature oocytcs curtured in MEM-fc medium containing FCS 5%, 7.5 IuhCG, 100mIUrFSH and group 3: 327 immature oocytes were growed in MEM-a medium FCS5%, 7.5 IUhCG, 100mIUrFSII, ImM glutathione. Fertilization and embryo development were done in T6 medium. Results : There were significant differences between in-vitro maturation rates (group 1: 59.71%, group 2: 79.28%, group 3: 52.74 and group 4: 70.22%) and resumpsion of meiosis (group 1: 74.20%, group 2: 92.88%, group 3: 74.26%, group 4: 86.20%) in different groups. Conclusion : The results of this study showed that glutathione (1mM) enhanced resumpsion of meiosis and in-vitro maturation and development of embryoes.