ARABIDOPSIS THALIANA گياه P5CS ژن رمزگردان آنزيم RNA از CDNA ساخت و تكثير

Production of cDNA from mRNA of P5CS Coding Gene in Arabidopsis thaliana

Plants encountered with drought and salinity produce osmolytes such as proline to help them overcome the stress. Proline is synthesized in a two-step pathway, in which A1-proline-5-carboxylate synthase (P5CS) is the key enzyme. To produce eDNA from mRNA , total RNA was extracted from leaves of Arabidopsis thaliana exposed to saline conditions. cDNA first strand was made through application of oligo (dT) primer and amplification of P5CS mRNA was carried out by two specifics, primer and polymerase chain reaction. Application of labeled probe, confirmed the amplification.