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EFFECTS OF MEDIA AND GROWTH REGULATORS ON GARDEN THYME (THYMUS VULGARIS L.) MICROPROPAGATION

Experiments were conducted to micropropagate the garden thyme (Thymus vulgaris L.) using stem single-node explants. Ten mm long stem explants were disinfested by 10% liquid bleach for 10 min and then rinsed three times with sterilized distilled water. Full and half-strength Murashige and Skoog (MS) media were compared. Both media were supplemented with benzyladenine (BA) and anaphthaleneacetic acid (NAA) at different concentrations. The best proliferation rate was obtained using medium containing 1 mg 1^-1 BA and 0.2 mg 1 NAA. In a separate experiment, the explants were four times subcultured on a medium containing best growth regulator concentrations of establishment stage with a 4-week interval. No significant differences were found between subcultures. For rooting, shoots were placed on MS medium supplemented with different concentrations of growth regulators, 25 g 1^-1 sucrose and 8 g 1^-1 agar. After 8 wk the best rooting treatment was application of 1.2.5 mg1^-1NAA without BA. Rooted plantlets were transferred to pasteurized media consisting a mixture of 1/6 peat, l/6 sand, 1/6 loam soil and 3/6 perlite. Plantlets were gradually acclimatized to natural conditions after 2 weeks.