پيوسته به ژن كنترل كننده تعداد رديف بذر در جو AFLP شناسايي نشانگرهاي

Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrsl) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrsl locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrsl locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrsl locus.