بررسي پروتيين هاي پادگني برخي سويه هاي SALMONELLA ABORTUS OVIS با استفاده از روش وسترن بلاتينگ

Antigenic characterization in Salmonella abortus ovis by western-blot method

Salmonella abortus ovis is the main causative agent of sheep abortion in Iran. Study of major antigens among different S.abortus ovis strains seems to be useful for designing the effective vaccine. In this experiment 37 S. abortus ovis strains were studied .Western- blot method has been applied to detect major antigens in S.ahortus ovis strains.All strains developed 5 and 3 major antigens respectively for SS44 and CH-Bakhtiary strains and Varamin strains. Antigens with 26, 15-20 and less than 9 kD yield more effective reaction with serum antibodies. Band of 26 kD was identical among all strains. Different S. abortus ovis strains revealed both identical and non identical antigens that not only could be used for further epidemiological studies but also assist designing of more effective vaccines for local and universal applications.