جداسازي ، كشت و بررسي گونه هاي اكتينوماسيز از بيماران مبتلا به عفونت فك تحتاني

Isolation and characterization of oral Actinomyces species from patients with periapical abscess

Background and Aim: The purpose of the study was isolation and characterization of the oral Actinomyces species believed to be the cause of the periapical abscesses. Methods and Materials: This epidemiological study was carried out on 32 patients with dento-alveolar abscesses, which have been referred to the Faculty of Dentistry, Tehran University of Medical Sciences. The specimens were collected during a period of 7 month, using aspiration method in the anaerobic condition. Samples were aseptically transferred to the individual screw bijoux bottles containing l ml Careerʹs Blair medium and sent to the laboratory within the next 30 minutes. Sample taking was performed from patients who had submandibular chronic dento-alveolar abscesses with no infectious diseases. An appropriate questionnaire was filled in for each case. Results: 32 specimens taken and one species of Actinomyces naeslundii was isolated from a patient with dentoalveolar abscess. Of the . 32 patients with dento-alveolar abscesses, aged between 14-47 years, 19 were male (59 4 %) and 13 were female (40.6 %). The highest incidence of the dento-alveolar abscess (25 %) was seen in the group aged 31-35 with the lowest incidence (6,3 %) was seen in the group aged 15-19 years. The causes of the demo-alveolar abscesses was found to be as follows: nineteen patients (59.4 %) with dental carries, seven patients (21.9 %) with a dental extraction and six patient (18.8 %) with uncompleted endodontic treatment. Conclusion: Results suggest that Actinomyces species may contribute to the etiology of the dento-alveolar abscesses.