تست تشخيص سريع مورفين در ادرار به روش مهار لاتكس آگلوتيناسيون

Rapid Latex Agglutination Inhibition Reaction Test for Morphine in Urine

A simple and rapid immunoassay of morphine based on latex agglutination inhibition reaction has been developed . Morphine-6-succinat is a morphine derivative , and its BSA conjugates were synthesized and characterized . The hapten density in the conjugate was determined spectroscopically to be 8.2 mol/mol of BSA . It was found that morphine-6-succinyl-BSA exhibits favorable features in terms of immunogenicity and immunochemical specificity . A latex agglutination inhibition reaction test (LAIRT) using DEAE-Cellulose purified rabbit IgG against morphine-6-succinyl-BSA was found to give a sensitivity of 300 mg/ml of morphine. Various commonly used drugs and narcotics at concentrations 0.1 mg/ml or less did not interfere with the test . Interfer.nce by normal urine was observed but it could be eliminated by the inclusion of 0.78% normal rabbit serum . The sensitized latex was stable at 4°C for at least 6 months . It was also stable to lyophilization and to at least four cycles of freezing and thawing . The total test time was 5 minutes . A comparision was made between the LAIRT and EMIT on 1 00 urine samples collected from people who refered to governmental narcotic clinic (Reference Lab. Tehran, Iran). While the EMIT showed 84 positives and 16 negatives , the LAIRT gave 68 positives and 32 negatives . The two tests showed no significant difference (P<0.05).