مطالعات ترموديناميكي و سينتيكي بر هم كنش آنزيم آلفا آميلاز ترموفيل و گوانيدين هيدروكلرايد

The interaction of guanidine hydrochloride and 77zerutophilic alpha-amylase (EC.3.2.1.1) from B.Subtilis was studied by the measurements of UV-Vis spectrophotometry and .specific activity. Spectral measurements were made at pH 69 and temperatures between 10 and 105 °C. It was observed that: (1) thermal stability of the enzyme is considerable; (2) enzyme stability increases in the presence of guanidine hydrochloride; (3) The enzyme looses its activity in presence of denaturant, and (4) in presence of guanidine hydrochloride Tyr and Trp are partially exposed.