بررسي دو پلي مورفيسم تاثيرگذار بر غلظت ليپوپروتئين با دانسيته بالا در بيماران قلبي عروقي با استفاده از روش واكنش زنجيره اي پليمراز- چند شكلي طول قطعه محدود (PCR-RFLP)

Investigation on two polymorphisms effective on HDL-C concentration in patients with coronary artery disease using restriction fragment length polymorphism

Background and aim: High density lipoprotein cholesterol (HOU) is a known inverse predictor of coronary heart disease (CHO). Cholesteryl ester transfer protein (CEIP) and hepatic lipase (HL) are key proteins in HOL-C metabolism so that decreased CEIP or HL activity is associated with high HOL-C. -fJ29CJA polymorphism in promoter ofCEIP gene and-514Cff in promoter 0 f HL gene were previously reported to reduce related protein level in plasma In this study association ofthese polymorphisms with COO related to OOL-C level were investigated Metbods: In this analytical-descriptive study 321 subjects underwent coronary angiography and divided in two groups base on angiogram (non CAD ~ 135 and CAD ~ 186). Serum lipids profile was measured by standard procedure and genotype was detected using PCR-RFLP method. Results: Overall the CETP genotype frequencies were in CAD patients: 58.8% (n=IIO), 28.9% (n~54) and 12.3% (n~23) and in non CAD patients: 45.2% (n~l), 41.5% (n~56) and 13.3% (n~18) for AA, CA and CC respectively. HL genotype frequencies were in CAD patients: 61.6% (n~114), 33.5% (n~2) and 4.9"10 (n~) and in non CAD patients: 65.9% (n~89), 27.4% (n~37) and 6.7% (n~) for CC, CT and IT respectively. In control group HDU concentration was higher for AA than CC genotype in -fJ29CIA, and also for IT than CC genotype in -514Ctr. Allele A in all subjects and T allele in woman were higher in CAD than non CAD group. A high increase in HDL-C level (10. mg/dl) was observed in individuals with CETP-AAlLIPC-IT and CETP-CAlL1PC-IT relative to CETP-CCILIPC-CC across all subjects (P< 0.00 I) but there was no difference in CAD prevalence. Conclusion: Allele A from -629CIA, and T from -514CIT even with the increasing of HDL-C concentration had higher frequency in CAD than non CAD group. Therefore, it seems that HDL-C didnʹt protect coronary artery when CETP or HL activity was reduced by these polymorphisms.