بررسي اختلالات رشدي و بازدارندگي رشد تنظيم كننده هاي پايريپروكسي فن و ديفلوبنزورون در شرايط حرارتي مختلف روي شب پره موم خوار بزرگ (Galleria mellonella L.)

Growth Disturbances and Inhibitory Effect of Pyriproxyfen and Diflubenzuron on the Greater Wax Moth (Galleria mellonella L.) under Different Temperatures

In this study diflubenzuron as a chitin synthesis inhibitor with a dosage of 25 mg A.I/L, and Pyriproxyfen as a juvenile hormone analogue with a dosage of 0.4 mgA.I/L were incorporated in the wax diets of young and last instar larvae of greater wax moth and reared at different temperatures (25, 30, 35°C). Results showed that these compounds in comparison with control increased larval mortality. Diflubenzuron and pyriproxyfen caused 33.9% and 24.1% mortality respectively. The young larvae showed higher mortality with the mean of 57.8% in diflubenzuron and 31.5% in pyriproxyfen. In evaluation of pupation and emergence of adults, no significant differences were observed between diflubenzuron and control. Pyriproxyfen induced reduction in pupation about 48.4% and adult emergence about 44.8% and caused some abnormalities of pupae. Last larval instar was more sensitive to JHA in this respect. Maximum pupation disruption occurred at 25°C and 30°C in comparison with 35°C which reduced abnormal pupae up to 19.7%. IGRs were effective in last larval instar at 35 °C and increased abnormality of adult up to 49.4%. Reproductive potential (egg viability, hatchability) of adults reduced in F1 generation and was 70-90 percent. IGRs have unfavorable effects on biological aspects of larvae; therefore, it is possible to use IGRs alone or in conjunction with other pesticides (Fumigants) in integrated control of these pests.