مطالعه تنوع آللي جايگاه ژني Glu-B3 در ارقام گندم زراعي ايران با استفاده از نشانگر مولكولي ALP (Amplicon Length Polymorphism)

Study of allelic variation at Glu-b3 locus of the Iranian bread wheat cultivars by using ALP molecular marker

Seed storage proteins of wheat grain are the most effective agents in bread making quality. Suitable dough should have high extensibility; therefore it is necessary to pay more attention to low molecular weigh (LMW) due to its role in dough extensibility. Since group B is the most important component of LMW and has the largest allelic variation, the polymorphism of this subunit was studied using ALP molecular marker. On the base of polymerase chain reaction (PCR), six groups of bands varying in length from 450-550bp were detected in using gel electrophoresis, indicating that allele variation in Iranian bread wheat cultivars. Among tested cultivars, the highest allele frequency belonged to r2 with 500bp length and the lowest allelic frequency was belonged to r3 with 470bp length. The results of cluster analysis showed that cultivars are put in three distinct groups. Relationship allelic variation at Glu-B3 and qualitative parameters show that cultivars with soft texture were completely lack of r3 band, whereas the presence of r2 band was quite evident in cultivars with soft texture.