مقايسه برخي پارامترهاي اسپرم شناختي و بيوشيميايي سمن ماهي سفيد مولد در زمان هاي مختلف مهاجرت توليد مثلي

Comparison of some spermatological and biochemical parameters of semen in Mahisefld {Rutilus frisii kutum) at different of broodstock spawning migration times

In this study effects of broodstock spawning migration time of Mahisefld {Rutilus frisii kutum) to Shiroud river on spermatological (sperm movement duration, sperm motility, spermatocrit, spermatozoa concentration and sperm volume) and biochemical (Na+, k+, Ca2+, Mg2+, pH, total protein, glucose and cholesterol) parameters of semen were investigated. For this goal the broodstock migration times were divided to 3 term and Samples was taked from 30 males. Sprem movement duration and spermatozoa motility (%) were was recorded by stero-microscope with camera connected to computer. The spermatocrit was measured by micro-hematocrit centrifuged. The sperm density was calculated with hemacytometer standard method (xlO9 per ml semen). pH was measured with pH meter. Na+ and K+ ions were measured by flame photometer, and Ca2+, Mg2+, glucose, cholesterol, total protein were measured by spectrophotometer. The result showed that sprem movement duration, spermatozoa motility (%) and K+ were significant (P<0.01) also spermatocrit, spermatozoa concentration, pH and Ca2+ concentration at the spawning migration times were significant (P<0.05). But sperm volume, Na+, Mg2+, glucose, cholesterol, total protein were not significant (P>0.05).