تنظيم پاسخهاي سيتوكايني در موشهاي آلوده به ليشمانياماژور با استفاده از ماده ايمونومدولاتور جداشده ازسير

Regulation of Th1/Th2 Cytokine Pattern in Leishmania Major Infected Balb/c Mice by an Immunomodulator Compound Isolated from Garlic

The regulation of Th1 and Th2 types of cytokine patterns is important in the final outcome of many diseases. The critical role of Thl and Th2 cytokine patterns is well characterized in the murine model of leishmaniasis. Balb/c mice are highly succeptible to Leishmania (L) major infection and develope a Th2-type response that is incapable of mediating parasite clearance and healing. We have examined the efficacy of garlic and an immunomodulator fraction isolated from garlic to promote healing and regulation of Th1/Th2 cytokine patterns in highly suscebtible Balb/c mice infected with L. major. Separate groups of infected mice received 20 mg/kg/day garlic, 0.4 mg/kg/day fraction or PBS alone (as control) on day 30 after infection for two weeks. ELISA was performed on spleen cell cultures supernatants for IFN-y, IL-2, IL-4 and IL-10. The results indicate that garlic and immunomodulator fraction treated mice developed Th1-type cytokine responses. However garlic is more effective than its isolated fraction far treatment of infection. These results suggest that garlic extract may provide effective therapy against L.major.