ISSR مقايسه جمعيت هاي كرم ابريشم چيني و بومي ايران با استفاده از نشانگرهاي

Comparison of China and Iranian native silkworm populations using ISSR markers

Two groups of silkworm (Bombyx mori-L) contain China (32, 104 and 110) and Iranian native silkworms (Guilan orange, Khorasan orange, Khorasan lemon, Khorasan pink and Bagdadi) were genetically compared. In this comparison ISSR markers were used with three primers. DNA was extracted from sericigene glands by modified Suzuki method (phenol-chloroform method). A set of three 5ʹ-anchored repeat primers amplified a total of 253 bands out of which %100 were polymorphic. Effective number of alleles average for total populations were 1.518 and genetic diversity average was 0.3194. A dendrogram was constructed using the UPGMA method. Maximum polymorphism was seen in 110 and minimum in lemon. Maximum unbiased genetic distance was between Gilani and 104 and minimum was between 1 10 and Baghdadi that were in one group. These results show that this marker is probably a useful tool for recognizing species, races, insectʹs biotype and phylogenetic studies.