توليد آنزيم زايلاناز بر روي ده نوع خوراك توسط قارچهاي بي هوازي شبكه

Production of zaylanase enzyme on ten feeds by rumen anaerobic fungi

An experiment was carried out to estimate the potential activity of rumen anaerobic fungi in production of zaylanase enzyme on different substrates. Samples of wheat straw, barley straw, wheat straw + urea, rice straw, wheat straw + molasses, wheat bran, bagasses (sugar cane), cotton seed, alfalfa and corn silage were used as the substrate to culture rumen fungi which isolated from a fistulated Sha] sheep. Zaylanase enzyme activities of samples were measured after 0, 3, 6 and 9 days of incubation. Production of zaylanase enzyme of substrates varied from 4.97 tol4.78 (unit enzyme) after 9 days of fungi growth. The highest and lowest production enzyme was related to wheat bran and barley straw, respectively. Enzyme production was increased sharply until 6 days of fungal incubation and continued slowly by 9th day in all of the cultures. The results indicated that rumen anaerobic fungi have the ability for enzyme production.