Protein adsorption on solid–liquid interfaces monitored by laser-ellipsometry

The adsorption of soluble proteins at solid–liquid interfaces plays a fundamental role in both, nature and industrial areas. The formation of organic molecular films below monolayer coverage can be resolved in real-time with ellipsometry, an optical technique based on the principle of change in state of polarization of light on reflection from an optically flat surface. In this communication studies of adsorption kinetics of the protein bovine serum albumin (BSA) on the substrates TiO2, Au and SiO2 are presented. The results show that the kinetics and degree of adsorption of BSA depend on the substrate material.