Abstract :
We present a novel laser-based approach for developing tissue engineered constructs and other cell-based assembly’s. We have deposited
mesoscopic patterns of viable B35 neuroblasts using a soft direct approach of the matrix assisted pulsed laser evaporation direct write (MAPLE
DW) process. As a development of the conventional direct write process, an intermediate layer of absorbing triazene polymer is used to provide
gentler and efficient transfers. Transferred cells were examined for viability and proliferation and compared with that of as-seeded cells to
determine the efficacy of the process. Results suggest that successful transfers can be achieved at lower fluences than usual by the incorporation of
the intermediate absorbing layer thus avoiding any damage to cells and other delicate materials. MAPLE DWoffers rapid computer-controlled
deposition of mesoscopic voxels at high spatial resolutions, with extreme versatility in depositing combinations of natural/synthetic, living/nonliving,
organic/inorganic and hard/soft materials. Our approach offers a gentle and efficient transfer of viable cells which when combined with a
variety of matrix materials allows development of constructs and bioactive systems in bioengineering
