Author/Authors :
Jagtap، G P نويسنده Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS). , , Mahajan، M V نويسنده Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS , , Utpal، D نويسنده Department of Plant Pathology, College of Agriculture, M.K.V., Parbhani – 431 402 (MS). ,
Abstract :
The polymerase Chain Reaction (PCR) diagnosis is more reliable and sensitive diagnostic tool for greening bacterium than other conventional approaches like Electron microscopy, DNA-DNA hybridization and immunofluorescence (IF) for detection of citrus greening. During experiment, it was observed that sodium sulphite method of DNA isolation provided higher yield and better quality DNA than other methods. Primer C (450 bp) was more efficient in amplifying the DNA of greening bacterium even at a very low concentration of 0.1 pg. To confirm the reliability of PCR, the greening bacterium was also detected in graft-inoculated plants, which showed typical greening bacterium was also detected in graft-inoculated plants, which showed typical greening symptoms. Results showed amplification of 450 bp in PCR suggesting sampling in March is more suitable for PCR detection of greening bacterium.
