Immobilization of naringinase on mesoporous molecular sieve MCM-41 and its application to debittering of white grapefruit

Naringinase was bound to mesoporous silica MCM-41 via adsorption with glutaraldehyde and used to debitter white grapefruit. Km value of the immobilized naringinase was lower than that of free naringinase. The immobilized catalysts showed excellent thermal stability and storage stability and could be recycled 6 times retained about 44.57% activities. The unaltered structural order of the prepared catalyst was characterized with reference to bulky and surface properties by infrared spectroscopy (FT-IR), elemental analysis and nitrogen adsorption–desorption isotherms analysis.