Author/Authors :
Vyas، S. نويسنده Department of Botany, University of Delhi, Delhi, India , , Kapoor-Pandey، P. نويسنده Department of Botany, University of Delhi, Delhi, India , , Guha، S.F. نويسنده Department of Botany, University of Delhi, Delhi, India , , Usha Rao، I. نويسنده Department of Botany, University of Delhi, Delhi, India ,
Abstract :
The present study was undertaken to investigate the role of banana extract
(BE) on synchronous plantlet formation from seeds of Dendrobium lituiflorum
Lindl. The seedlings formed on modified Knudson C (KC) medium supplemented
with 20% (v/v) BE had significantly high fresh and dry weights. Morphologically
uniform plantlets with elongated leaves and well developed roots were formed
on KC medium with 20% (v/v) BE in comparison to KC medium without BE
(control) after 30 d of fourth subculture. The plantlets so formed were
subjected to in vitro hardening on agar-agar gelled medium, Luffa sponge and
cocopeat: perlite (9:1) as support matrices, each containing one-half strength
KC major salts and were successfully acclimatized under greenhouse conditions.
Banana extract helps in synchronous plantlet formation in vitro which is not
only beneficial for conservation purposes but also to the biotech industries as
a large number of uniform plantlets can be obtained for transplantation,
thereby, reducing the cost of production.
