Author/Authors :
Kavitha، M.S. نويسنده Centre for Biotechnology, Muthayammal College of Arts & Science, Rasipuram-637408, Tamil Nadu, India. , , Wesely، E.G. نويسنده PG Department of Botany, A.A. Government Arts College, Namakkal, Tamil Nadu, India. , , Mehalingam، P. نويسنده Research Centre in Botany, V.H.N. Senthikumara Nadar College, Virudhunagar, Tamil Nadu India. ,
Abstract :
In vitro multiple shoot regeneration of Solanum nigrum L., an Indian
medicinal plant was accomplished on MS medium utilizing shoot tip and
nodal explants. Direct multiple shoots differentiated within 6 weeks when
explants were cultured on MS medium containing BAP (1.0 – 5.0 mg/l) and
KIN (1.0 – 5.0 mg/l) individually. Among various concentrations of cytokinins
tested, maximum number of multiple shoots was obtained on MS medium
supplemented with BAP (1.0 mg/l) from shoot tip (20.4 ± 0.22) and MS
medium supplemented with BAP (3.0 mg/l) from nodal explants (8.4 ± 0.22).
The in vitro regenerated shoots were rooted (8.4 ± 0.16 roots per shoot) on MS
medium supplemented with NAA (1.0 mg/l) within 2-3 weeks of culture and
the regenerated plantlets could be successfully established in soil where they
grow normally.
