Correlation of Serum ALP Activity with the Healing Process of Femoral Fractures in Rats Used Cissus Quadrangularis Extract as Therapy

The purpose of this study was to find out corellate value of serum ALP activity with the process of fracture healing in rat with therapy Cissus quadrangularis extract. This study used 18 male white rats (Rattus Norwegicus) age of 12 weeks. After 1 week of adaptation, all rats were treated femoral dexter osteotomy under aseptic conditions. Then, reposition of femoral dexter fractures with intramedullary pin fixation. The rats model were evenly randomly divided into three groups at 2nd weeks after surgery: group I treated with normal diet, group II with normal diet + CaCo3 supplement 150 mg/day, group III with normal diet + Cissus qudrangularis extract 500 mg / day per oral. Furthermore, the treatment was carried out for 4 weeks. At the end of the experiment (6th weeks after surgery), the blood samples were taken directly from canthus orbitalis dexter for alkaline phosphatase analysis. The data were analyzed by One Way Anova using SPSS for Windows program. The results indicated that ALP activity was significantly decreased ( p < 0,05). The more complete the fracture healing process, ALP activity the closer to normal value. The conclusion is the serum total ALP actvity have a positive correlation with the process of fracture healing and could be used to determine the progress of fracture healing of the surgically treated fractures.