Author/Authors :
Masaru Okuda، نويسنده , , Henning F Horn، نويسنده , , Pheruza Tarapore، نويسنده , , Yukari Tokuyama، نويسنده , , A.George Smulian، نويسنده , , Pui-Kwong Chan، نويسنده , , Erik S. Knudsen، نويسنده , , Irene A Hofmann، نويسنده , , Jean D Snyder، نويسنده , , Kevin E. Bove، نويسنده , , Kenji Fukasawa، نويسنده ,
Abstract :
In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E–mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.
