Author/Authors :
Melissa R. van Dijk، نويسنده , , Chris J. Janse، نويسنده , , Joanne Thompson، نويسنده , , Andrew P. Waters، نويسنده , , Joanna A.M. Braks، نويسنده , , Huub J. Dodemont، نويسنده , , Henk G. Stunnenberg، نويسنده , , Geert-Jan van Gemert، نويسنده , , Robert W. Sauerwein، نويسنده , , Wijnand Eling، نويسنده ,
Abstract :
Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45− parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45− parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.
