Author/Authors :
Safitri ، E. نويسنده Department of Veterinary Reproduction Faculty of Veterinary Med, Airlangga University. , , Utama ، S. نويسنده Department of Veterinary Reproduction Faculty of Veterinary Med, Airlangga University. , , Bumi ، C. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Mulyani ، S. W. M. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Susilowati ، H. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Retnowati ، E. نويسنده Department of Clinical Pathology, Faculty of Medicine, Airlangga University. , , Purwati.، - نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Prasetyo ، R. H. نويسنده Departement of Parasitology School of Medicine, Airlangga University. , , Hariadi ، M. نويسنده Department of Veterinary Reproduction Faculty of Veterinary Med, Airlangga University. , , Rantam ، F. A. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. ,
Abstract :
The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).
