Author/Authors :
Haixiao Gao and Joachim Frank، نويسنده , , Jayati Sengupta، نويسنده , , Mikel Valle، نويسنده , , Andrei Korostelev، نويسنده , , Narayanan Eswar، نويسنده , , Scott M. Stagg، نويسنده , , Patrick Van Roey، نويسنده , , Rajendra K. Agrawal، نويسنده , , Stephen C. Harvey، نويسنده , , Andrej Sali، نويسنده , , Michael S. Chapman، نويسنده , , Joachim Frank، نويسنده ,
Abstract :
Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
