Author/Authors :
Mike Boxem، نويسنده , , Zoltan Maliga، نويسنده , , Niels Klitgord، نويسنده , , Na Li، نويسنده , , Irma Lemmens، نويسنده , , Miyeko Mana، نويسنده , , Lorenzo de Lichtervelde، نويسنده , , Joram D. Mul، نويسنده , , Diederik van de Peut، نويسنده , , Maxime Devos، نويسنده , , Nicolas Simonis، نويسنده , , Muhammed A. Yildirim، نويسنده , , Murat Cokol، نويسنده , , Huey-Ling Kao، نويسنده , , Anne-Sophie de Smet، نويسنده , , Haidong Wang، نويسنده , , Anne-Lore Schlaitz، نويسنده , , Tong Hao، نويسنده , , Stuart Milstein، نويسنده , , Changyu Fan، نويسنده , , et al.، نويسنده ,
Abstract :
Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or “interactome” networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
