Author/Authors :
Jeong Beom Kim، نويسنده , , Vittorio Sebastiano، نويسنده , , Guangming Wu، نويسنده , , Marcos J. Arauzo-Bravo، نويسنده , , Philipp Sasse، نويسنده , , Luca Gentile، نويسنده , , Kinarm Ko، نويسنده , , David Ruau، نويسنده , , Mathias Ehrich، نويسنده , , Dirk van den Boom، نويسنده , , Johann Meyer، نويسنده , , Karin Hübner، نويسنده , , Christof Bernemann، نويسنده , , Claudia Ortmeier، نويسنده , , Martin Zenke، نويسنده , , Bernd K. Fleischmann، نويسنده , , Holm Zaehres، نويسنده , , Hans R. Sch?ler، نويسنده ,
Abstract :
The four transcription factors Oct4, Sox2, Klf4, and c-Myc can induce pluripotency in mouse and human fibroblasts. We previously described direct reprogramming of adult mouse neural stem cells (NSCs) by Oct4 and either Klf4 or c-Myc. NSCs endogenously express Sox2, c-Myc, and Klf4 as well as several intermediate reprogramming markers. Here we report that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs. These one-factor induced pluripotent stem cells (1F iPS) are similar to embryonic stem cells in vitro and in vivo. Not only can these cells can be efficiently differentiated into NSCs, cardiomyocytes, and germ cells in vitro, but they are also capable of teratoma formation and germline transmission in vivo. Our results demonstrate that Oct4 is required and sufficient to directly reprogram NSCs to pluripotency.
