Author/Authors :
Jorge Z. Torres، نويسنده , , Matthew K. Summers، نويسنده , , David Peterson، نويسنده , , Matthew J. Brauer، نويسنده , , James Lee، نويسنده , , Silvia Senese، نويسنده , , Ankur A. Gholkar، نويسنده , , Yu-Chen Lo، نويسنده , , Xingye Lei، نويسنده , , Kenneth Jung، نويسنده , , David C. Anderson، نويسنده , , David P. Davis، نويسنده , , Lisa Belmont، نويسنده , , Peter K. Jackson، نويسنده ,
Abstract :
During cell division, cells form the microtubule-based mitotic spindle, a highly specialized and dynamic structure that mediates proper chromosome transmission to daughter cells. Cancer cells can show perturbed mitotic spindles and an approach in cancer treatment has been to trigger cell killing by targeting microtubule dynamics or spindle assembly. To identify and characterize proteins necessary for spindle assembly, and potential antimitotic targets, we performed a proteomic and genetic analysis of 592 mitotic microtubule copurifying proteins (MMCPs). Screening for regulators that affect both mitosis and apoptosis, we report the identification and characterization of STARD9, a kinesin-3 family member, which localizes to centrosomes and stabilizes the pericentriolar material (PCM). STARD9-depleted cells have fragmented PCM, form multipolar spindles, activate the spindle assembly checkpoint (SAC), arrest in mitosis, and undergo apoptosis. Interestingly, STARD9-depletion synergizes with the chemotherapeutic agent taxol to increase mitotic death, demonstrating that STARD9 is a mitotic kinesin and a potential antimitotic target.
