Title of article :
Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells
Author/Authors :
Hui Yang، نويسنده , , Linyu Shi، نويسنده , , Bang-An Wang، نويسنده , , Dan Liang، نويسنده , , Cuiqing Zhong، نويسنده , , Wei Liu، نويسنده , , Yongzhan Nie، نويسنده , , Jie Liu، نويسنده , , Jing Zhao، نويسنده , , Xiang Gao، نويسنده , , Dangsheng Li، نويسنده , , GUOLIANG XU، نويسنده , , Jinsong Li، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2012
Pages :
13
From page :
605
To page :
617
Abstract :
Haploid cells are amenable for genetic analysis. Recent success in the derivation of mouse haploid embryonic stem cells (haESCs) via parthenogenesis has enabled genetic screening in mammalian cells. However, successful generation of live animals from these haESCs, which is needed to extend the genetic analysis to the organism level, has not been achieved. Here, we report the derivation of haESCs from androgenetic blastocysts. These cells, designated as AG-haESCs, partially maintain paternal imprints, express classical ESC pluripotency markers, and contribute to various tissues, including the germline, upon injection into diploid blastocysts. Strikingly, live mice can be obtained upon injection of AG-haESCs into MII oocytes, and these mice bear haESC-carried genetic traits and develop into fertile adults. Furthermore, gene targeting via homologous recombination is feasible in the AG-haESCs. Our results demonstrate that AG-haESCs can be used as a genetically tractable fertilization agent for the production of live animals via injection into oocytes.
Journal title :
CELL
Serial Year :
2012
Journal title :
CELL
Record number :
1021162
Link To Document :
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