Title of article :
Cell-free Formation of RNA Granules: Bound RNAs Identify Features and Components of Cellular Assemblies
Author/Authors :
Tina W. Han، نويسنده , , Masato Kato، نويسنده , , Shanhai Xie، نويسنده , , Leeju C. Wu، نويسنده , , Hamid Mirzaei، نويسنده , , Jimin Pei، نويسنده , , Min Chen، نويسنده , , Yang Xie، نويسنده , , Jeffrey Allen، نويسنده , , Guanghua Xiao، نويسنده , , Steven L. McKnight، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2012
Pages :
12
From page :
768
To page :
779
Abstract :
Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mRNAs contain extended 3′ UTR sequences and an enrichment in binding sites for known granule-associated proteins. Hydrogels composed of the low complexity (LC) sequence domain of FUS recruited and retained the same mRNAs as were selectively precipitated by the b-isox chemical. Phosphorylation of the LC domain of FUS prevented hydrogel retention, offering a conceptual means of dynamic, signal-dependent control of RNA granule assembly.
Journal title :
CELL
Serial Year :
2012
Journal title :
CELL
Record number :
1021177
Link To Document :
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