Author/Authors :
Georgina M. Ellison، نويسنده , , Carla Vicinanza، نويسنده , , Andrew J. Smith، نويسنده , , Iolanda Aquila، نويسنده , , Angelo Leone، نويسنده , , Cheryl D. Waring، نويسنده , , Beverley J. Henning، نويسنده , , Giuliano Giuseppe Stirparo، نويسنده , , Roberto Papait، نويسنده , , Marzia Scarf?، نويسنده , , Valter Agosti، نويسنده , , Giuseppe Viglietto، نويسنده , , Gianluigi Condorelli، نويسنده , , Ciro Indolfi، نويسنده , , Sergio Ottolenghi، نويسنده , , Daniele Torella، نويسنده , , Bernardo Nadal-Ginard، نويسنده ,
Abstract :
The epidemic of heart failure has stimulated interest in understanding cardiac regeneration. Evidence has been reported supporting regeneration via transplantation of multiple cell types, as well as replication of postmitotic cardiomyocytes. In addition, the adult myocardium harbors endogenous c-kitpos cardiac stem cells (eCSCs), whose relevance for regeneration is controversial. Here, using different rodent models of diffuse myocardial damage causing acute heart failure, we show that eCSCs restore cardiac function by regenerating lost cardiomyocytes. Ablation of the eCSC abolishes regeneration and functional recovery. The regenerative process is completely restored by replacing the ablated eCSCs with the progeny of one eCSC. eCSCs recovered from the host and recloned retain their regenerative potential in vivo and in vitro. After regeneration, selective suicide of these exogenous CSCs and their progeny abolishes regeneration, severely impairing ventricular performance. These data show that c-kitpos eCSCs are necessary and sufficient for the regeneration and repair of myocardial damage.
