Author/Authors :
Sara Cogliati، نويسنده , , Christian Frezza، نويسنده , , Maria Eugenia Soriano، نويسنده , , Tatiana Varanita، نويسنده , , Ruben Quintana-Cabrera، نويسنده , , Mauro Corrado، نويسنده , , Sara Cipolat، نويسنده , , Veronica Costa، نويسنده , , Alberto Casarin، نويسنده , , Ligia C. Gomes، نويسنده , , Ester Perales-Clemente، نويسنده , , Leonardo Salviati، نويسنده , , Patricio Fernandez-Silva، نويسنده , , Jose A. Enriquez، نويسنده , , Luca Scorrano، نويسنده ,
Abstract :
Respiratory chain complexes assemble into functional quaternary structures called supercomplexes (RCS) within the folds of the inner mitochondrial membrane, or cristae. Here, we investigate the relationship between respiratory function and mitochondrial ultrastructure and provide evidence that cristae shape determines the assembly and stability of RCS and hence mitochondrial respiratory efficiency. Genetic and apoptotic manipulations of cristae structure affect assembly and activity of RCS in vitro and in vivo, independently of changes to mitochondrial protein synthesis or apoptotic outer mitochondrial membrane permeabilization. We demonstrate that, accordingly, the efficiency of mitochondria-dependent cell growth depends on cristae shape. Thus, RCS assembly emerges as a link between membrane morphology and function.
