Amperometric glucose sensor using tetrathiafulvalene in Nafion gel as electron shuttle

An amperometric mediated sensor for glucose has been contrived by using bovine serum albumin and glutaraldehyde to immobilize glucose oxidase on a Nafion-tetrathiafulvalene (TTF) modified electrode. It is further coated by Nafion. The inner Nafion membrane can prevent leaking of tetrathiafulvalene; the outer Nafion film serves as a barrier to electroactive anionic interferents such as ascorbate and urate and protects the biosensor from fouling agents. The experiment shows that TTF+ and TTF2+ can oxidize the reduced flavin adenine dinucleotide (FADH2) of glucose oxidase. The biosensor responds to glucose in less than 50 s and its calibration curve is linear from 3.0 × 10−4 to l.0 × 10−2 M.