Screen-printed amperometric biosensors for glucose and alcohols based on ruthenium-dispersed carbon inks

A ruthenium-dispersed carbon ink is used for the fabrication of screen-printed enzyme electrodes. The dispersed ruthenium particles offer an efficient electrocatalytic action towards the detection of enzymatically-liberated peroxide and dihydronicotin-amide adenine dinucleotide (NADH). Highly selective biosensing of glucose is accomplished at a potential region (0.0 to + 0.2 V) where interfering reactions are minimized. Similarly, the metallized strip surface (with co-immobilized alcohol dehydrogenase and NAD+) facilitates the low-potential biosensing of ethanol without the assistance of redox mediators.