Application of SIMPLISMA for the assessment of peak purity in liquid chromatography with diode array detection

The application of SIMPLISMA for the investigation of peak purity with liquid chromatography and diode array detection (LC-DAD) is proposed. SIMPLISMA is applied in the Chromatographic direction, and is used to detect pure zones in the chromatogram. The performance of SIMPLISMA and of an approach based on the Gram-Schmidt orthogonalization technique are compared.