Amperometric glucose biosensor manufactured by a printing technique

A glucose sensor was fabricated using the screen printing technique with a special ink component containing ferrocene-bound glucose oxidase (fc-GOD). Glucose oxidase was modified with ferrocene derivatives as the electron-transfer mediator in order to obtain higher GOD activity. The ferrocene-modified GOD showed an increase in catalytic current. A special ink component compatible with the printing process was prepared with fc-GOD, soluble and insoluble polymer resins and organic solvent. Using the conventional screen printing technique, the ink component was printed on a thin gold film electrode on a plastic film. The output of the printed glucose sensor was observed to be stable for several hours and proportional to the glucose concentration from 0.5 to 2.0 mg ml−1.