Title of article
Cotranscriptional Set2 Methylation of Histone H3 Lysine 36 Recruits a Repressive Rpd3 Complex
Author/Authors
Grunstein، Michael نويسنده , , Boone، Charles نويسنده , , Weissman، Jonathan S. نويسنده , , Schuldiner، Maya نويسنده , , Collins، Sean R. نويسنده , , Thompson، Natalie J. نويسنده , , Punna، Thanuja نويسنده , , Greenblatt، Jack F. نويسنده , , Krogan، Nevan J. نويسنده , , Keogh، Michael-Christopher نويسنده , , Kurdistani، Siavash K. نويسنده , , Morris، Stephanie A. نويسنده , , Ahn، Seong Hoon نويسنده , , Podolny، Vladimir نويسنده , , Chin، Kayu نويسنده , , Emili، Andrew نويسنده , , Hughes، Timothy R. نويسنده , , Strahl، Brian D. نويسنده , , Buratowski، Stephen نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2005
Pages
-592
From page
593
To page
0
Abstract
The yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and geneexpression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Rpd3C(S) mutants exhibit phenotypes remarkably similar to those of Set2, a histone methyltransferase associated with elongating RNA polymerase II. Chromatin immunoprecipitation and biochemical experiments indicate that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2.
Keywords
DIGLYPHUS ISAEA , Liriomyza trifolii , Abamectin compatibility , Biological control , IPM , Greenhouse
Journal title
CELL
Serial Year
2005
Journal title
CELL
Record number
102330
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