Determination of fat-soluble vitamins by capillary liquid chromatography in bovine blood plasma

The fat-soluble vitamins retinyl palmitate, D3, E and K1 were separated on a packed reversed-phase fused-silica capillary column using methanol-tetrahydrofuran as mobile phase, and determined with UV detection. Conventional liquid Chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system. The vitamins could be separated isocratically with detection limits of 2.9 pg, 1 pg, 29 pg and 2.6 pg, respectively. Application of the proposed method to the quantification of fat-soluble vitamins in blood is reported.