Enantiomeric separation of six 2-arylpropionic acids after pre-column derivatization with various amines and alcohols on a cellulose-based chiral stationary phase

A large number of mono-, bi- and tricyclic amines and alcohols were used for the derivatization of six 2-arylpropionic acids. The effect of the bonded aromatic portion on the enantiomeric separation using a tris(4-methylbenzoate)cellulose column was studied. Small modifications of the bonded groups close to the chiral centre pointed out their predominant steric effect to the chiral recognition process rather than electronic influence. Varying ratios of methanol and perchlorate buffer composing the mobile phase were applied to elute the different series within comparable analysis times.